We have a substance with several molecules, and we want to separate them by size. We want to collect molecules of the same size in a container so that we can analyse and characterise them.
The mixture is mixed with a solvent (e.g. chloroform). Then up to 10 ml is drawn into a syringe and injected into the instrument. If the amount of mixture is larger, the automatic injection option can be used. In this case, the instrument draws the substance directly from a container.
The separation principle works based on molecular mass. Heavy molecules whiz through the tubes faster, while lighter molecules hit the edges of the tubes more often and therefore march through the tubes more slowly. In this way, molecules with different masses can be separated.
It takes about 15 minutes to pass through one column, and about 30 minutes to pass through two columns. The more often these passes take place, the cleaner the separation.
The injection site is then rinsed with the same solvent to remove impurities.
A sample is supplied by the manufacturer for use as a reference sample after installation.
(Written by Monika Martin, PHI GmbH, Marketing & Communications)